CIE A Level Biology复习笔记3.2.7 Enzyme Inhibitors
Enzyme Inhibitors An enzyme's activity can be reduced or stopped, temporarily, by a reversible inhibitor There are two types of reversible inhibitors: Competitive inhibitors have a similar shape to...
Enzyme Inhibitors An enzyme's activity can be reduced or stopped, temporarily, by a reversible inhibitor There are two types of reversible inhibitors: Competitive inhibitors have a similar shape to...
Vmax & the Michaelis-Menten Constant Substrate concentration affects the rate of catalysis in an enzyme-substrate reaction When the substrate concentration is fixed (and enzyme concentration is...
Rate: Inhibitor Concentration There are two types of inhibitors: Competitive inhibitors have a similar shape to that of the substrate molecules and therefore compete with the substrate for the acti...
Rate: Enzyme Concentration Enzyme concentration affects the rate of reaction The higher the enzyme concentration in a reaction mixture, the greater the number of active sites available and the grea...
Rate: pH All enzymes have an optimum pH or a pH at which they operate best Enzymes are denatured at extremes of pH Hydrogen and ionic bonds hold the tertiary structure of the protein (ie. the enzym...
Rate: Temperature Enzymes have a specific optimum temperature – the temperature at which they catalyse a reaction at the maximum rate Lower temperatures either prevent reactions from proceeding or ...
Colorimetry to Measure Enzyme Activity A colorimeter is able to measure light absorbance (how much light is absorbed) or light transmission (how much light passes through) a substance Colorimetry c...
Measuring Enzyme Activity The progress of enzyme-catalysed reactions can be investigated by: Measuring the rate of formation of a product using catalase Measuring the rate of disappearance of a sub...
How Enzymes Work The lock-and-key hypothesis Enzymes are globular proteins This means their shape (as well as the shape of the active site of an enzyme) is determined by the complex tertiary struct...
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