CIE A Level Biology复习笔记19.1.2 Genetic Engineering

• Genetic engineering is a technique used to deliberately modify a specific characteristic (or characteristics) of an organism. The technique involves removing a gene (or genes) with the desired characteristic from one organism and transferring the gene (using a vector) into another organism where the desired gene is then expressed
• The genetically engineered organism will then contain recombinant DNA and will be a genetically modified organism (GMO)
• In order for an organism to be genetically engineered the following steps must be taken:
  • Identification of the desired gene
  • Isolation of the desired gene by:
    • Cutting from a chromosome using enzymes (restriction endonucleases)
    • Using reverse transcriptase to make a single strand of complementary DNA (cDNA) from mRNA
    • Creating the gene artificially using nucleotides
  • Multiplication of the gene (using polymerase chain reaction - PCR)
  • Transfer into the organism using a vector (e.g. plasmids, viruses, liposomes)
  • Identification of the cells with the new gene (by using a marker), which is then cloned
• Genetic engineers need the following to modify an organism:
  • Enzymes (restriction endonucleases, ligase and reverse transcriptase)
  • Vectors - used to deliver genes into a cell (eg. plasmids, viruses and liposomes)
  • Markers - genes that code for identifiable substances that can be tracked (eg. GFP - green fluorescent protein which fluoresces under UV light or GUS - β-glucuronidase enzyme which transforms colourless or non-fluorescent substrates into products that are coloured or fluorescent)
• Genetic engineering is being used in the new field of science called synthetic biology
  • This is an area of research that studies the design and construction of different biological pathways, organisms and devices, as well as the redesigning of existing natural biological systems

An overview of the steps taken to genetically engineer an organism (in this case bacteria are being genetically engineered to produce human insulin)