AQA A Level Chemistry复习笔记7.11.5 Interpreting Chromatograms

• The less polar components travel further up the TLC plate
  • Their Rf values are higher than those closer to the baseline
  • They are more soluble in the mobile phase and get carried forwards with the solvent

   

• More polar components do not travel far up the plate
  • They are more attracted to the polar stationary phase

   

• The extent of separating molecules in the investigated sample depends on the solubility in the mobile and stationary phases
• Knowing the Rf values, of compounds being analysed, helps to compare the polarity of various molecules

Answers:

Blue spot Rf value = 2.82 ÷ 4.63=  0.61 = Leucine

Green spot Rf value = 2.45 ÷ 4.63= 0.536 = Isoleucine

Red spot Rf value = 2.04  ÷ 4.63= 0.44 = Valine